446 research outputs found

    Revisiting the Cellulosimicrobium cellulans yeast-lytic β-1,3-glucanases toolbox: A review

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    Cellulosimicrobium cellulans (also known with the synonyms Cellulomonas cellulans, Oerskovia xanthineolytica, and Arthrobacter luteus) is an actinomycete that excretes yeast cell wall lytic enzyme complexes containing endo-β-1,3-glucanases [EC 3.2.1.39 and 3.2.1.6] as key constituents. Three genes encoding endo-β-1,3-glucanases from two C. cellulans strains have been cloned and characterised over the past years. The βglII and βglII(A )genes from strain DSM 10297 (also known as O. xanthineolytica LL G109) encoded proteins of 40.8 and 28.6 kDa, respectively, whereas the β-1,3-glucanase gene from strain ATCC 21606 (also known as A. luteus 73–14) encoded a 54.5 kDa protein. Alignment of their deduced amino acid sequences reveal that βglII and βglII(A )have catalytic domains assigned to family 16 of glycosyl hydrolases, whereas the catalytic domain from the 54.5 kDa glucanase belongs to family 64. Notably, both βglII and the 54.5 kDa β-1,3-glucanase are multidomain proteins, having a lectin-like C-terminal domain that has been assigned to family 13 of carbohydrate binding modules, and that confers to β-1,3-glucanases the ability to lyse viable yeast cells. Furthermore, βglII may also undergo posttranslational proteolytic processing of its C-terminal domain, resulting in a truncated enzyme retaining its glucanase activity but with very low yeast-lytic activity. In this review, the diversity in terms of structural and functional characteristics of the C. cellulans β-1,3-glucanases has been compiled and compared

    Reflexiones éticas sobre las enfermedades raras y los medicamentos huérfanos

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    Exposición a la pornografía y su influencia en la asertividad sexual

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    Treball Final de Grau en Psicologia. Codi: PS1048. Curs: 2019/2020Pornography has been more available since the arrival of the Internet, broadband networks and smartphones. Pornography exposure may lead to negative psychological and behavioral consequences. Besides, sexual assertion plays a role against risky sexual behaviour, sexual abuse and victimization. The aim of this study is to analyze the relation between wanted and unwanted exposure to pornography and sexual assertion.La pornografía se ha vuelto más accesible desde la llegada de internet, la banda ancha y los teléfonos inteligentes. La exposición a la pornografía puede tener consecuencias psicológicas y conductuales negativas. Por otra parte, la asertividad sexual actúa como factor de protección ante conductas sexuales de riesgo, abusos y victimización sexual. El objetivo del presente trabajo es analizar la relación entre la voluntaria e involuntaria exposición a la pornografía y la asertividad sexual

    Mathematical modeling of nitrogen regulated biological systems

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    Mención Internacional en el título de doctorThis doctoral thesis was supported through the FPI contract BES-2017-079755 associated to the grant FIS2016-78313-P from Ministerio de Ciencia e Innovación (MCIN)/Agencia Estatal de Investigación (AEI)/ 10.13039/501100011033/ and FEDER Una manera de hacer Europa. Additionally the author also acknowledge finantial support from MCIN/AEI/10.13039/501100011033 through grant BADS, no. PID2019-109320GB-100Programa de Doctorado en Ingeniería Matemática por la Universidad Carlos III de MadridPresidente: Luis Guillermo Morelli.- Secretaria: María Pilar Guerrero Contreras.- Vocal: Vicente Mariscal Romer

    Design and manufacturing of a raceway pond reactor through FFF process

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    In automotive projects always, it is possible to optimize the cost to obtain an economic prize for unit. For that reason, constantly perform studies to improve the aspect that effects to the final value directly. In this project has been detailed the methodology that has been used in SEAT, also in the Group Volkswagen, to initiate a new automotive project. It is possible to remark three different parts in this document: The first one refers to the introduction of the company and the collaboration with Volkswagen, explaining how was been created the union between them and the history and evolution of both companies In the second part, it has detailed the objective of the project and the premises and what are the guidelines that should be taken into account to work in the same direction of the Group. It is also explained the methodology decided to initiate this project, depending on the peculiarities on it, and define how will structure the teamwork and how will administrate the resources to develop the different implicated concepts. The last part of the document shows the obtained results in each decided action, detailing in which phase of the project it is and the comparison with passed projects used as reference

    Els arxius familiars i patrimonials aranesos: documents per a l'estudi de la Societat de la Val d'Aran

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    The Archiu Generau d'Aran holds forty-six family and heritage archives from the Vall D'Aran towns of Bausen, Canejan, Les, Bossòst, Arres de Sus, Arres de Jos, Vilamòs, Arró, Es Bòrdes, Benós, Arròs, Vilac, Vielha, Betren, Garòs, Arties, Salardú, Unha, Tredòs and Bagergue. To a lesser or greater extent, all these archives contain a series of documents that provide a view of the tangible and intangible assets of these families. The family archives contain documents related to members from same family and their legal affairs, especially marriage contracts, wills and dowries. Heritage archives have documents related to private property, which changed hands using a range of instruments. The Arxiu de çò de Joanchiquet (1509-1971) contains family, heritage and personal documentation for the Aunòs family in Vilamòs. This includes documents on their family alliances, inheritances, businesses and lists of family members in government offices in the Val d'Aran. A library with over four hundred volumes completes this archive, one of the most important conserved at the Archiu Generau d'Aran.A l'Archiu Generau d'Aran es troben dipositats quaranta-sis arxius familiars i patrimonials dels pobles aranesos de Bausen, Canejan, Les, Bossòst, Arres de Sus, Arres de Jos, Vilamòs, Arró, Es Bòrdes, Benós, Arròs, Vilac, Vielha, Betren, Garòs, Arties, Salardú, Unha, Tredòs i Bagergue. En tots aquests arxius es troben, en major o menor quantitat, una sèrie de documents que donen una visió de quin és el patrimoni material i immaterial d'aquestes famílies. La documentació familiar va lligada a la mateixa família i als seus negocis jurídics, dels quals destaquen el capítol matrimonial, el testament i el dot. La documentació patrimonial va relacionada amb la propietat particular, que canvia de mans a través de diversos instruments. L'arxiu de çò de Joanchiquet (1509-1971) recull la documentació familiar, patrimonial i personal de la família Aunòs de Vilamòs. En ell es conserven documents sobre les seves aliances familiars, el seu patrimoni, els seus negocis i les relacions dels seus membres en els oficis que regeixen l'administració de la Val d'Aran. Una biblioteca amb més de quatre-cents volums completa aquest arxiu, un dels més importants que es conserven a l'Archiu Generau d'Aran

    Era Val d'Aran e és sòns privilègis

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